I-Olivetol, eyaziwa nangokuthi i-5-pentylresorcinol, iyinhlanganisela eyenzeka ngokwemvelo esithole ukunakwa okukhulu eminyakeni yamuva nje ngokusetshenziswa kwayo kwezemithi kanye nezimboni.Kuyi-molecule eyandulelayo ye-biosynthesis yezinhlanganisela ezihlukahlukene, kuhlanganise nama-cannabinoids atholakala ngokuyinhloko esitshalweni se-cannabis.Ukuqonda i-biosynthesis yei-olivetolkubalulekile ukubona amandla ayo futhi uhlole izinhlelo zayo ezihlukahlukene.
Ukwakhiwa kwe-biosynthesisI-Olivetoliqala ngokufingqa kwama-molecule amabili e-malonyl-CoA, atholakala ku-acetyl-CoA, ngokusebenzisa isenzo se-enzyme ebizwa nge-polyketide synthase.Lokhu kusabela kwe-condensation kuholela ekwakhekeni kwenhlanganisela ephakathi ebizwa ngokuthi i-geranyl pyrophosphate, okuyisandulela esivamile ku-biosynthesis yemikhiqizo ehlukahlukene yemvelo, kuhlanganise ne-terpenes.
I-Geranyl pyrophosphate ibe isiguqulwa ibe i-olive acid ngochungechunge lwe-enzymatic reactions.Isinyathelo sokuqala sibandakanya ukudlulisa iqembu le-isoprenyl lisuka ku-gerynyl pyrophosphate liye ku-molecule ye-hexanoyl-CoA, kwakha inhlanganisela ebizwa nge-hexanoyl-CoA olive acid cyclase.Lokhu kusabela kwe-cyclization kwenziwa yi-enzyme ebizwa nge-hexanoyl-CoA:olivelate cyclase.
Isinyathelo esilandelayo sokungenai-olivetoli-biosynthesis ihlanganisa ukuguqulwa kwe-hexanoyl-CoA olivetate cyclase ibe ifomu elisebenzayo elibizwa ngokuthi i-tetraketide intermediate.Lokhu kufezwa ngochungechunge lokusabela kwe-enzymatic okubangelwa ama-enzyme afana ne-chalcone synthase, i-stilbene synthase, ne-resveratrol synthase.Lokhu kusabela kuholela ekwakhekeni kwe-tetraketide intermediate, ebese iguqulelwa ku-olivetol ngesenzo se-polyketide reductase.
Kanyei-olivetolihlanganiswa, ingabuye iguqulwe ibe yizinhlanganisela ezihlukahlukene, kuhlanganise nama-cannabinoids, ngokusebenzisa isenzo sama-enzyme afana ne-cannabidiolic acid synthase kanye ne-delta-9-tetrahydrocannabinolic acid synthase.Lawa ma-enzyme adala ukufiphala kwe-condensationi-olivetolnge-geranyl pyrophosphate noma amanye ama-molecule angaphambili ukwenza ama-cannabinoids ahlukene.
Ngaphezu kwendima yayo ku-cannabinoid biosynthesis,i-olivetolkutholakale ukuthi inezinto ezingaba yi-antifungal kanye ne-antioxidant.Ucwaningo luye lwabonisa lokhoi-olivetolingavimbela ukukhula kwezinhlobonhlobo ze-fungal pathogens, okwenza kube ikhandidethi elithembisayo lokuthuthukiswa kwezidakamizwa ze-antifungal.Ngaphezu kwalokho,i-olivetolkukhonjiswe ukuthi inomsebenzi onamandla wokuqothula ngokumelene nama-radicals mahhala, okungama-molecule asebenza kakhulu angabangela ukulimala kwamangqamuzana nezicubu.Le mpahla ye-antioxidant yei-olivetoliphakamisa ukusetshenziswa kwayo okungase kube khona ekuthuthukiseni ama-ejenti okwelapha ekwelapheni izifo ezihlobene nokucindezeleka okwenziwe nge-oxidative.
Ngamafuphi, i-biosynthesis yei-olivetolkuhilela ukujiya kwama-molecule e-malonyl-CoA, okulandelwa uchungechunge lokusabela kwe-enzymatic, okuholela ekwakhekenii-olivetol.Le nhlanganisela isebenza njenge-molecule eyandulelayo ku-biosynthesis yama-cannabinoids kanye neminye imikhiqizo yemvelo.Ukuqonda indlela ye-biosynthetic yeI-Olivetolkubalulekile ekuthuthukiseni izinhlelo zayo ezingase zibe khona emikhakheni yezemithi nezimboni.Ucwaningo olwengeziwe ku-biosynthesis yei-olivetolkanye nokuphuma kwayo kungase kuholele ekutholakaleni kwezinhlanganisela ezintsha zokwelapha kanye nosizo ekwakhiweni kwemithi emisha.
Isikhathi sokuthumela: Nov-13-2023